2O8J
Human euchromatic histone methyltransferase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.586, 70.965, 83.046 |
| Unit cell angles | 81.11, 75.13, 88.39 |
Refinement procedure
| Resolution | 79.310 - 1.800 |
| R-factor | 0.18385 |
| Rwork | 0.182 |
| R-free | 0.22124 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2igq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 79.300 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.048 |
| Number of reflections | 100323 |
| <I/σ(I)> | 13.7 |
| Completeness [%] | 92.2 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | peg 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
