2O8H

Crystal structure of the catalytic domain of rat phosphodiesterase 10A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-05-22
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	120.622, 120.622, 82.143
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.227
Rwork	0.224
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology model of PDE10a
RMSD bond length	0.017
RMSD bond angle	1.476
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.063	0.038	0.675
Number of reflections	37865
<I/σ(I)>	12.2
Completeness [%]	91.4	97.5	53.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1