2O7I
The X-ray crystal structure of a thermophilic cellobiose binding protein bound with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.030, 107.030, 118.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.740 - 1.500 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vr5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 | |
| High resolution limit [Å] | 1.500 | 6.000 | 1.500 |
| Rmerge | 0.062 | 0.023 | 0.578 |
| Number of reflections | 109675 | ||
| <I/σ(I)> | 18.8 | 61.6 | 3 |
| Completeness [%] | 99.9 | 100 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1M MES, 0.2M Ammonium Sulfate, 30% PEG MME 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
