2O7H
Crystal structure of trimeric coiled coil GCN4 leucine zipper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.051, 56.256, 54.863 |
| Unit cell angles | 90.00, 129.05, 90.00 |
Refinement procedure
| Resolution | 42.600 - 1.860 |
| R-factor | 0.18128 |
| Rwork | 0.179 |
| R-free | 0.23097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zij |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.162 | |
| Number of reflections | 15146 | |
| <I/σ(I)> | 7.59 | |
| Completeness [%] | 98.8 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 30% 2-Proponal, 0.2M Sodium Citrate, 0.1M Sodium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
