2O70
Structure of OHCU decarboxylase from zebrafish
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.07225 |
Spacegroup name | P 32 |
Unit cell lengths | 101.815, 101.815, 103.915 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.000 - 1.800 |
R-factor | 0.19777 |
Rwork | 0.196 |
R-free | 0.24022 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.167 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.690 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.072 | 0.409 |
Number of reflections | 111211 | |
<I/σ(I)> | 17.4 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | 20 % (v/v) EtOH, 100mM TrisHCl, pH 8.5, VAPOR DIFFUSION, temperature 277K |
1 | VAPOR DIFFUSION | 8.5 | 277 | 20 % (v/v) EtOH, 100mM TrisHCl, pH 8.5, VAPOR DIFFUSION, temperature 277K |