2O6Y
Tyrosine ammonia-lyase from Rhodobacter sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.925, 155.626, 164.352 |
Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
Resolution | 500.000 - 1.500 |
Rwork | 0.174 |
R-free | 0.18900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gkm: Histidine ammonia-lyase from Pseudomonas putida |
RMSD bond length | 0.004 |
RMSD bond angle | 1.175 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.085 | 0.618 |
Number of reflections | 652406 | |
<I/σ(I)> | 12.2 | 2.3 |
Completeness [%] | 93.3 | 85.9 |
Redundancy | 5.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M MOPSO (pH 7.0), 7% (w/v) polyethylene glycol 8000, 0.3 M ammonium acetate, 2 mM dithiothreitol, 35 mM cyclohexylbutanoyl-N-hydroxyethylglucamide., VAPOR DIFFUSION, HANGING DROP, temperature 277K |