2O6W
Crystal Structure of a Pentapeptide Repeat Protein (Rfr23) from the cyanobacterium Cyanothece 51142
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 |
Unit cell lengths | 106.391, 106.391, 52.419 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
Rwork | 0.251 |
R-free | 0.26230 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 16261 | |
<I/σ(I)> | 42.7 | 4.53 |
Completeness [%] | 89.3 | 61.7 |
Redundancy | 10.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.192 magnesium acetate, 0.096M sodium cacodylate, 10%(w/v) PEG 8000, 10%(w/v) glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |