2O6U
Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- new crystal form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 93.099, 93.099, 85.734 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.850 - 3.010 |
| R-factor | 0.19677 |
| Rwork | 0.195 |
| R-free | 0.23843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2av9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.842 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.086 | 0.499 |
| Number of reflections | 8614 | |
| <I/σ(I)> | 23.4 | 2.5 |
| Completeness [%] | 97.8 | 98.9 |
| Redundancy | 8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
