2O6T
Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (P2221).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 58.924, 97.083, 191.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 2.550 |
| R-factor | 0.18341 |
| Rwork | 0.180 |
| R-free | 0.24788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2av9 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.740 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.121 | 0.508 |
| Number of reflections | 36563 | |
| <I/σ(I)> | 16.5 | 2.3 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 6.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 3350, O.1M Bis-Tris pH 5.5, 0.5% NDSB-201, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
