2O6S
Structural diversity of the hagfish Variable Lymphocyte Receptors B59
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2006-07-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97175 |
Spacegroup name | P 1 |
Unit cell lengths | 30.657, 50.027, 69.642 |
Unit cell angles | 69.21, 76.99, 86.12 |
Refinement procedure
Resolution | 19.820 - 1.500 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o6r |
RMSD bond length | 0.006 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 58682 |
<I/σ(I)> | 28.7 |
Completeness [%] | 91.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 33% PEG 1000, 0.1M Bis-Tris, 0.2M Sodium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |