2O6P
Crystal Structure of the heme-IsdC complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 277 |
| Collection date | 2006-08-31 |
| Wavelength(s) | 0.933, 1.739, 1.741, 1.722 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.489, 70.441, 88.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.990 - 1.500 |
| R-factor | 0.19487 |
| Rwork | 0.193 |
| R-free | 0.22204 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.030 | 0.430 |
| Number of reflections | 45711 | |
| <I/σ(I)> | 12.3 | 1.8 |
| Redundancy | 4.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | IsdC at 95mg/ml in 50mM Tris-HCl pH 8.0, 500mM NaCl mixed in equal volumes (2 + 2 l) with reservoir solution containing 26% PEG 550, MME, 18mM zinc sulphate, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
