2O6N
RH4B: designed right-handed coiled coil tetramer with all biological amino acids
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-28 |
| Wavelength(s) | 1.000,1.2398,1.3858,1.3864 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 38.356, 38.356, 49.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.360 - 1.100 |
| R-factor | 0.116 |
| Rwork | 0.115 |
| R-free | 0.13200 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.360 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.083 | 0.011 |
| Number of reflections | 20631 | |
| <I/σ(I)> | 21.09 | 1.26 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 10% PEG 4000, 0.1M ammonium sulfate, 0.15M sodium acetate, 10mM ytterbium(II) chloride, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
