2O6F
Structure of metal- free rTp34 from Treponema pallidum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-14 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97899 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.251, 66.034, 151.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.030 - 1.630 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.21900 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | native protein |
RMSD bond length | 0.015 |
RMSD bond angle | 1.893 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.030 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 41967 | |
<I/σ(I)> | 32 | 2.2 |
Completeness [%] | 95.3 | 94.1 |
Redundancy | 4.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 2.4 M Ammonium sulfate, 0.1 M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |