2O6E
Structure of native rTp34 from Treponema pallidum from zinc-soaked crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.28230 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.451, 65.921, 151.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.600 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | native protein |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.962 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 29534 | |
| <I/σ(I)> | 19.5 | 1.8 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 2.4 M Ammonium sulfate, 0.1 M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
