2O6C
Structure of selenomethionyl rTp34 from Treponema pallidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-22 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.410, 66.020, 150.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.530 - 1.700 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.530 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 38808 | |
| <I/σ(I)> | 39.8 | 1.9 |
| Completeness [%] | 99.5 | 93.3 |
| Redundancy | 14.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 2.4 M Ammonium sulfate, 0.1 M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
