2O67
Crystal structure of Arabidopsis thaliana PII bound to malonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-21 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.594, 66.726, 61.489 |
| Unit cell angles | 90.00, 118.41, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.20401 |
| Rwork | 0.201 |
| R-free | 0.26567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o66 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.039 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.104 | 0.517 |
| Number of reflections | 11464 | |
| <I/σ(I)> | 14.2 | 2.4 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.5 M malonate, 0.1 M Na HEPES, pH 7.0, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
