2O5R
Crystal structure of Glutamyl-tRNA synthetase 1 (EC 6.1.1.17) (Glutamate-tRNA ligase 1) (GluRS 1) (TM1351) from Thermotoga maritima at 2.5 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.94926, 0.97925, 0.97939 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 69.953, 69.953, 283.945 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.351 - 2.340 |
R-factor | 0.236 |
Rwork | 0.234 |
R-free | 0.26800 |
Structure solution method | MAD, MOLECULAR REPLACEMENT |
Starting model (for MR) | 1G59 Chain A |
RMSD bond length | 0.013 |
RMSD bond angle | 1.427 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.351 | 47.400 | 2.420 |
High resolution limit [Å] | 2.340 | 5.020 | 2.340 |
Rmerge | 0.165 | 0.012 | 0.012 |
Number of reflections | 35284 | ||
<I/σ(I)> | 6.6 | 14.9 | 1.6 |
Completeness [%] | 99.9 | 99.6 | 100 |
Redundancy | 6.57 | 7.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 8 | 277 | 0.2M CaAcetate, 10.0% PEG-8000, 0.1M Imidazole pH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |