2O5P
Crystal structure of the full length ferric pyoverdine outer membrane receptor FpvA of Pseudomonas aeruginosa in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.975650 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.880, 128.780, 139.150 |
| Unit cell angles | 90.00, 130.55, 90.00 |
Refinement procedure
| Resolution | 30.470 - 2.770 |
| R-factor | 0.20976 |
| Rwork | 0.208 |
| R-free | 0.24724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iah |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.388 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.470 | 2.920 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Number of reflections | 63018 | |
| <I/σ(I)> | 13.7 | 2.7 |
| Completeness [%] | 97.5 | 83.7 |
| Redundancy | 3.93 | 2.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M MES, 1.3-1.4 M Na2HPO4, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
