2O5N

Crystal structure of a Viral Glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	61.969, 78.178, 62.726
Unit cell angles	90.00, 90.43, 90.00

Refinement procedure

Resolution	62.750 - 2.400
R-factor	0.233
Rwork	0.230
R-free	0.27900
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.318
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.750	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.081	0.404
Number of reflections	26191
<I/σ(I)>	9.7	1.54
Completeness [%]	97.5	80.6
Redundancy	12.8	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	25 % PEG 2000 MME, 0.1M Tris-HCl, 0.5 % n-dodecyl-b-D-maltoside, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	25 % PEG 2000 MME, 0.1M Tris-HCl, 0.5 % n-dodecyl-b-D-maltoside, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K