2O5H
Uncharacterized Protein Conserved in Bacteria, COG3792 from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-22 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.822, 67.153, 93.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.610 - 1.900 |
| R-factor | 0.2063 |
| Rwork | 0.201 |
| R-free | 0.24917 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.446 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.610 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.627 |
| Number of reflections | 24290 | |
| <I/σ(I)> | 6.7 | 2.17 |
| Completeness [%] | 99.5 | 96.3 |
| Redundancy | 15.5 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.8M Sodium phosphate, 1.2M Potassium phosphate, 0.1M Sodium acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
