2O57
Crystal Structure of a putative sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97923, 0.96400 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.541, 291.433, 86.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.771 - 1.946 |
R-factor | 0.168 |
Rwork | 0.166 |
R-free | 0.22100 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.452 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 48.771 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.110 | 0.066 | 0.597 |
Number of reflections | 89709 | ||
<I/σ(I)> | 7.618 | 1.775 | |
Completeness [%] | 99.4 | 99.6 | 95 |
Redundancy | 7.2 | 7.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein Solution (10 MG/ML protein, 0.050 M sodium chloride, 0.0031 M sodium azide, 0.0003 M TCEP, 0.005 M Bis Tris pH 7.0) mixed in a 1:1 ratio with the Well Solution (23% MEPEG 5K, 0.03 M sarcosine, 0.1 M MOPS pH 7.0), Cryoprotected with: well solution supplemented with up to 15% glycerol, vapor diffusion, hanging drop, temperature 293K |