2O50
The crystal structure of Toxoplasma gondii Enoyl acyl carrier protein reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.542 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 76.004, 76.004, 187.471 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.2128 |
Rwork | 0.209 |
R-free | 0.27732 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o2y |
RMSD bond length | 0.012 |
RMSD bond angle | 1.389 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.180 | 0.650 |
Number of reflections | 13758 | |
<I/σ(I)> | 16.2 | 4.5 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 0.1M Tris, 25% (v/v) tert-Butanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |