2O4W
T4 lysozyme circular permutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.118, 60.118, 96.531 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.17646 |
| Rwork | 0.175 |
| R-free | 0.21288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sx7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.359 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.098 | 0.396 |
| Number of reflections | 16286 | |
| <I/σ(I)> | 10.4 | 2.8 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | 0.9 M K2HPO4, 1.1 M NaH2PO4, pH 6.9, 0.25 M NaCl, 49.0 mM oxidized B-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
