2O4V
An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97965, 0.97983, 0.96441 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 255.130, 142.769, 62.025 |
Unit cell angles | 90.00, 99.49, 90.00 |
Refinement procedure
Resolution | 62.900 - 1.940 |
R-factor | 0.17528 |
Rwork | 0.174 |
R-free | 0.19397 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.154 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 62.900 |
High resolution limit [Å] | 1.940 |
Rmerge | 0.067 |
Number of reflections | 147021 |
<I/σ(I)> | 14.8 |
Completeness [%] | 91.1 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Hepes pH 6.5, 50mM K2HPO4, 5% MPD, 7.5-10% PEG8K, VAPOR DIFFUSION, SITTING DROP, temperature 291K | |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Hepes pH 6.5, 50mM K2HPO4, 5% MPD, 7.5-10% PEG8K, VAPOR DIFFUSION, SITTING DROP, temperature 291K |