2O4U
Crystal structure of Mammalian Dimeric Dihydrodiol Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 123.177, 123.177, 121.127 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.000 |
| R-factor | 0.16306 |
| Rwork | 0.161 |
| R-free | 0.19333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o48 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.334 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | |
| Number of reflections | 37154 | |
| <I/σ(I)> | 17.9 | 11.77 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 1.5M ammonium phosphate, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
