2O4I
Structure of TREX1 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.758, 80.758, 171.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.850 - 3.500 |
| R-factor | 0.24516 |
| Rwork | 0.243 |
| R-free | 0.28335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o4g |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.007 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.580 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 6708 | |
| <I/σ(I)> | 3.7 | 1.1 |
| Completeness [%] | 87.9 | 83.6 |
| Redundancy | 6.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 2KMME, 0.1M Imidazole, 0.3M lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2KMME | ||
| 2 | 1 | 1 | Imidazole | ||
| 3 | 1 | 1 | lithium sulfate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 2KMME | ||
| 6 | 1 | 2 | lithium sulfate | ||
| 7 | 1 | 2 | H2O |
