2O4D
Crystal Structure of a hypothetical protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-12 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 92.718, 92.718, 65.303 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.090 | 0.446 |
| Number of reflections | 14543 | |
| <I/σ(I)> | 28.5 | 9.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.5 | 20.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 298 | 0.2M NaCl, 0.1M Tris pH7.6, 0.4M sodium dihydrogen phosphate, 1.6M dipotassium hydrogen phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
