2O4D
Crystal Structure of a hypothetical protein from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-12 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 92.718, 92.718, 65.303 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.850 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.20600 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.219 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.090 | 0.446 |
Number of reflections | 14543 | |
<I/σ(I)> | 28.5 | 9.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 21.5 | 20.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 298 | 0.2M NaCl, 0.1M Tris pH7.6, 0.4M sodium dihydrogen phosphate, 1.6M dipotassium hydrogen phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |