2O4A
Crystal Structure of the N-terminal CUT Domain of SATB1 Bound to Matrix Attachment Region DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.659, 38.318, 40.709 |
| Unit cell angles | 71.34, 82.50, 66.63 |
Refinement procedure
| Resolution | 22.290 - 1.750 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o49 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.040 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.290 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.094 | 0.275 |
| Number of reflections | 14958 | |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 86.3 | 55.2 |
| Redundancy | 2.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 20000, 0.05M TrisHCl, 0.01M magnesium sulfate, 20% ethylene glycol, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 20000 | ||
| 2 | 1 | 1 | TrisHCl | ||
| 3 | 1 | 1 | magnesium sulfate | ||
| 4 | 1 | 1 | ethylene glycol | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | PEG 20000 | ||
| 7 | 1 | 2 | TrisHCl | ||
| 8 | 1 | 2 | magnesium sulfate | ||
| 9 | 1 | 2 | HOH |
