2O49
Crystal Structure of the N-terminal CUT domain of SATB1 Bound to Matrix Attachment Region DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-01-16 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.659, 36.997, 41.248 |
| Unit cell angles | 71.60, 83.92, 71.07 |
Refinement procedure
| Resolution | 19.570 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yse |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.080 |
| Data reduction software | SAINT (PLUS) |
| Data scaling software | SCALA |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.640 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.155 |
| Number of reflections | 9632 | |
| <I/σ(I)> | 21.6 | 3.9 |
| Completeness [%] | 89.8 | 55.2 |
| Redundancy | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 20000, 0.05M TrisHCl, 0.01M magnesium chloride, 20% ethylene glycol, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 20000 | ||
| 2 | 1 | 1 | TrisHCl | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | ethylene glycol | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | PEG 20000 | ||
| 7 | 1 | 2 | TrisHCl | ||
| 8 | 1 | 2 | magnesium chloride | ||
| 9 | 1 | 2 | HOH |
