2O48
Crystal structure of Mammalian Dimeric Dihydrodiol Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 122.853, 122.853, 121.664 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.590 |
| R-factor | 0.16344 |
| Rwork | 0.160 |
| R-free | 0.22776 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.889 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 17024 | |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 98.1 | 95.6 |
| Redundancy | 3.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 2M ammonium sulphate, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
