2O42
Crystal Structure of M11L, Bcl-2 homolog from myxoma virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-08 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 73.151, 73.151, 186.875 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.910 |
R-factor | 0.25114 |
Rwork | 0.249 |
R-free | 0.29000 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.966 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 13415 | |
<I/σ(I)> | 22.92 | 4.596 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.6 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | sodium citrate, ammonium sulfate, KCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | sodium citrate, ammonium sulfate, KCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | sodium citrate, ammonium sulfate, KCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |