2O3Y

Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site in Presence of Paromamine Derivative NB30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8730
Spacegroup name	P 21 21 2
Unit cell lengths	47.940, 49.230, 57.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
Rwork	0.245
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fqn
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.380	37.380	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.086	0.037	0.387
Total number of observations		1285	1336
Number of reflections	3818
<I/σ(I)>	7.8	20.6	2.7
Completeness [%]	94.7	90	96.7
Redundancy	3.4	3.09	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	Sodium Cacodylate, Potassium chloride, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, spermine tetrahydrochloride , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Sodium Cacodylate
10	1	2	spermine tetrahydrochloride
2	1	1	Potassium chloride
3	1	1	2-methyl-2,4-pentanediol
4	1	1	hexammine cobalt chloride
5	1	1	spermine tetrahydrochloride
6	1	2	Sodium Cacodylate
7	1	2	Potassium chloride
8	1	2	2-methyl-2,4-pentanediol
9	1	2	hexammine cobalt chloride