2O3W
Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site in presence of paromomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 47.537, 50.310, 57.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.350 - 2.800 |
Rwork | 0.251 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fqn |
RMSD bond length | 0.006 |
RMSD bond angle | 0.990 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.397 | 57.400 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.085 | 0.060 | 0.414 |
Total number of observations | 611 | 3428 | |
Number of reflections | 3672 | ||
<I/σ(I)> | 5 | 8.4 | 1.6 |
Completeness [%] | 99.8 | 92.1 | 100 |
Redundancy | 6.2 | 4.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | Sodium Cacodylate, Potassium chloride, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, spermine tetrahydrochloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium Cacodylate | ||
2 | 1 | 1 | Potassium chloride | ||
3 | 1 | 1 | 2-methyl-2,4-pentanediol | ||
4 | 1 | 1 | hexammine cobalt chloride | ||
5 | 1 | 1 | spermine tetrahydrochloride | ||
6 | 1 | 2 | Potassium chloride | ||
7 | 1 | 2 | 2-methyl-2,4-pentanediol | ||
8 | 1 | 2 | hexammine cobalt chloride | ||
9 | 1 | 2 | spermine tetrahydrochloride |