2O3I
X-ray Crystal Structure of Protein CV_3147 from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvR68.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.336, 125.608, 65.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.920 - 2.300 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.114 | 0.473 |
| Number of reflections | 65131 | |
| <I/σ(I)> | 14.3 | 4.9 |
| Completeness [%] | 96.9 | 97.1 |
| Redundancy | 6.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 9 | 277 | 40% PEG 1000, 0.1 M Magnesium sulfate, 0.1 M TAPS, pH 9.0, Microbatch under oil, temperature 277K |
