2O38
Putative XRE Family Transcriptional Regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-06 |
Detector | SBC-3 |
Wavelength(s) | 0.97904 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.994, 58.276, 83.459 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.700 - 1.830 |
R-factor | 0.20861 |
Rwork | 0.202 |
R-free | 0.27050 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.505 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.700 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.064 | 0.435 |
Number of reflections | 15203 | |
<I/σ(I)> | 8.4 | 1.8 |
Completeness [%] | 99.1 | 93.6 |
Redundancy | 11.6 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 2M Sodium formate, 0.1M Sodium acetate, pH 4.6, 0.3M PPS, VAPOR DIFFUSION, HANGING DROP, temperature 295K |