2O38
Putative XRE Family Transcriptional Regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.994, 58.276, 83.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.700 - 1.830 |
| R-factor | 0.20861 |
| Rwork | 0.202 |
| R-free | 0.27050 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.505 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.064 | 0.435 |
| Number of reflections | 15203 | |
| <I/σ(I)> | 8.4 | 1.8 |
| Completeness [%] | 99.1 | 93.6 |
| Redundancy | 11.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 2M Sodium formate, 0.1M Sodium acetate, pH 4.6, 0.3M PPS, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
