2O2Z

Crystal structure of a protein member of the upf0052 family (bh3568) from bacillus halodurans at 2.60 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-31
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	51.430, 143.640, 233.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.437 - 2.600
R-factor	0.19
Rwork	0.188
R-free	0.22400
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.500
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.437	29.400	2.690
High resolution limit [Å]	2.600	5.570	2.600
Rmerge	0.083	0.029	0.401
Number of reflections	54381
<I/σ(I)>	15.41	35	2.9
Completeness [%]	99.6	98.8	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	8.5	277	40.0% polyethylene glycol 400, 0.2M lithium sulfate, 0.1M TRIS pH 8.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K