2O2X
Crystal structure of a putative had-like phosphatase (mll2559) from mesorhizobium loti at 1.50 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.480, 66.270, 41.020 |
| Unit cell angles | 90.00, 108.53, 90.00 |
Refinement procedure
| Resolution | 28.262 - 1.500 |
| R-factor | 0.139 |
| Rwork | 0.138 |
| R-free | 0.16700 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.262 | 1.550 | |
| High resolution limit [Å] | 1.500 | 2.560 | 1.500 |
| Rmerge | 0.046 | 0.034 | 0.271 |
| Number of reflections | 29534 | ||
| <I/σ(I)> | 11.11 | 19.9 | 2.88 |
| Completeness [%] | 94.8 | 96.7 | 88.8 |
| Redundancy | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 6.64 | 277 | 28.5% polyethylene glycol 6000, 0.1M HEPES pH 6.64, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
