2O2T
The crystal structure of the 1st PDZ domain of MPDZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.366, 32.660, 92.803 |
Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
Resolution | 29.780 - 2.700 |
R-factor | 0.22681 |
Rwork | 0.224 |
R-free | 0.29131 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.250 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.780 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.078 | 0.513 |
Number of reflections | 8029 | |
<I/σ(I)> | 1.93 | |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.5% Jeffamine 2001, 1.1M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |