2O2J
Mycobacterium tuberculosis tryptophan synthase beta chain dimer (apoform)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.05 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.823, 114.966, 159.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.560 |
R-factor | 0.21441 |
Rwork | 0.210 |
R-free | 0.30376 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o2e |
RMSD bond length | 0.039 |
RMSD bond angle | 3.112 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.630 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.047 | 0.367 |
Number of reflections | 22608 | |
<I/σ(I)> | 13.5 | 2 |
Completeness [%] | 96.9 | 93.4 |
Redundancy | 2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Hepes, 2.0 M Ammonium Formate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |