2O2B
Spectroscopic and Structural Study of the Heterotropic Linkage between Halide and Proton Ion Binding to Gfp Proteins: E2(GFP)-I Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.066, 62.854, 69.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.660 - 1.940 |
| R-factor | 0.18416 |
| Rwork | 0.182 |
| R-free | 0.23422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h6v |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.636 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.499 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.130 | 0.438 |
| Number of reflections | 16918 | |
| <I/σ(I)> | 12.8 | 2.9 |
| Completeness [%] | 98.2 | 91.1 |
| Redundancy | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 14% (W/V) PEG 3350, 100 MM NH4 ACETATE, 0.2 M NH4I, PH 5.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 100K |
