2O2A
The crystal structure of a protein of unknown function from Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 103.110, 103.110, 278.408 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.850 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.25232 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.501 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.155 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.119 | 0.690 |
| Number of reflections | 31918 | |
| <I/σ(I)> | 25.66 | 2.2 |
| Completeness [%] | 99.8 | 99.71 |
| Redundancy | 13.8 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100 mM Sodium Acetate trihydrate, 3 M Sodium Chloride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
