2O1X
1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-25 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.261, 154.062, 124.881 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.382 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.114 | 0.420 |
| Number of reflections | 64098 | |
| <I/σ(I)> | 13.4 | 3.3 |
| Completeness [%] | 97.4 | 96.6 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM Tris, pH7.5, 200 mM ammonium acetate, 150 mM NaCl, and 20% (w/v) PEG 6000 or PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
