2O1V

Structure of full length GRP94 with ADP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	99.080, 108.850, 148.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.25015
Rwork	0.248
R-free	0.29947
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.356
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.450	2.450
Number of reflections	59644
<I/σ(I)>	15.6	2
Completeness [%]	99.6	97.7
Redundancy	4.8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	26-32% v/v PEG400, 150-225mM MgCl2, and 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	26-32% v/v PEG400, 150-225mM MgCl2, and 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	26-32% v/v PEG400, 150-225mM MgCl2, and 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K