2O1U
Structure of full length GRP94 with AMP-PNP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.330, 109.260, 148.887 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.890 - 2.400 |
| R-factor | 0.24537 |
| Rwork | 0.243 |
| R-free | 0.28888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GRP94(73-754d41) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.890 | 2.350 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.082 | 0.504 |
| Number of reflections | 79363 | |
| <I/σ(I)> | 22.6 | 3.1 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 12.4 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 26-32% v/v PEG400, 150-225mM MgCl2, and 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
