2O1T
Structure of Middle plus C-terminal domains (M+C) of GRP94
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 131.300, 129.540, 184.780 |
Unit cell angles | 90.00, 99.91, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.28492 |
Rwork | 0.284 |
R-free | 0.29373 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.307 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 100423 | |
<I/σ(I)> | 14 | 2 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 5.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 13-18% v/v 2-methyl-2,4-pentanediol (MPD), 2-5% w/v PEG 6000, and 0.1M bis-tris, pH 6.0 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 13-18% v/v 2-methyl-2,4-pentanediol (MPD), 2-5% w/v PEG 6000, and 0.1M bis-tris, pH 6.0 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 13-18% v/v 2-methyl-2,4-pentanediol (MPD), 2-5% w/v PEG 6000, and 0.1M bis-tris, pH 6.0 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |