2O1P
Structure of yeast Poly(A) Polymerase in a somewhat closed state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.217, 108.587, 132.718 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.510 - 2.700 |
R-factor | 0.19892 |
Rwork | 0.195 |
R-free | 0.27508 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fa0 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.318 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.306 |
Number of reflections | 31665 | |
<I/σ(I)> | 16.9 | 5.2 |
Completeness [%] | 99.3 | 97.7 |
Redundancy | 10.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 20% Peg 8000 100mM magnesium acetate 100mM imidazole pH 6.2 3% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |