2O1N

Crystal structure of a complex of phospholipase A2 with a peptide Ala-Ile-Ala-Ser at 2.8 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	292
Detector technology	IMAGE PLATE
Collection date	2006-11-26
Detector	MAR scanner 345 mm plate
Spacegroup name	P 43
Unit cell lengths	52.960, 52.960, 48.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.730 - 2.800
R-factor	0.188
Rwork	0.188
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sv3
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.840
High resolution limit [Å]	2.800	2.800
Number of reflections	3224
Completeness [%]	94.7	82.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	PEG 4000, 0.2 M Ammonium Sulphate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K