2O1K
Structure of the extended diarrhea-inducing domain of rotavirus enterotoxigenic protein NSP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 30.692, 38.090, 182.354 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.670 |
| R-factor | 0.18973 |
| Rwork | 0.188 |
| R-free | 0.23318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 1G1J |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.117 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.045 | 0.142 |
| Number of reflections | 12801 | |
| <I/σ(I)> | 29.8 | 12.5 |
| Completeness [%] | 98.8 | 95.5 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5M lithium sulphate monohydrate, 0.1 HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
