2O1H
Naturally occurring mutation of Humna ABO(H) Galactosyltransferase in complex with UDP: GTB/M214T_UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-27 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.600, 149.490, 79.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.670 |
R-factor | 0.17835 |
Rwork | 0.176 |
R-free | 0.22193 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lz7 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.852 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CCP4 ((MOLREP)) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.730 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.054 | 0.299 |
Number of reflections | 36659 | |
<I/σ(I)> | 15.2 | 4.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.71 | 6.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | ADA, manganese chloride, ammonium sulfate, MPD, glycerol, PEG 4000,, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |