2O1E
Crystal Structure of the Metal-dependent Lipoprotein YcdH from Bacillus subtilis, Northeast Structural Genomics Target SR583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-20 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.292, 54.344, 62.840 |
| Unit cell angles | 103.70, 100.46, 96.75 |
Refinement procedure
| Resolution | 19.930 - 2.600 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.058 | 0.277 |
| Number of reflections | 35170 | |
| <I/σ(I)> | 11.5 | 2.22 |
| Completeness [%] | 94.3 | 88.7 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM Tris-HCl, 40% PEG1k, 100 mM magnesium acetate, and 5 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
